Fig. 4

The protective function of si-circ-PDE1C was suppressed by miR-766-3p inhibitor in IL-1β-treated CHON-001 cells. CHON-001 cells were treated with control, IL-1β, IL-1β + si-NC, IL-1β + si-circ-PDE1C, IL-1β + si-circ-PDE1C + inhibitor NC or IL-1β + si-circ-PDE1C + miR-766-3p inhibitor. A CCK-8 assay was used to measure cell proliferation. B Flow cytometry was used to assess cell apoptosis. C Western blotting assay was used to examine the protein expression of PCNA, Cleaved caspase-3 and Aggrecan. D ELISA was used to determine the levels of TNF-α and IL-6. E–G ROS level (E), MDA level (F) and SOD activity (G) were detected by commercial kits. *P < 0.05, **P < 0.01, ***P < 0.001