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Fig. 3 | Advances in Rheumatology

Fig. 3

From: Circ-PDE1C/miR-766-3p/SGTB axis regulates the IL-1β-induced apoptosis, inflammation and oxidative stress in human chondrocytes

Fig. 3

Circ-PDE1C was confirmed as a miR-766-3p inhibitor. A The binding sites between circ-PDE1C and miR-766-3p were predicted using circinteractome. B The overexpression efficiency of miR-766-3p mimic was determined using RT-qPCR. C, D The combination between circ-PDE1C and miR-766-3p was validated using dual-luciferase reporter assay (C) and RIP assay (D). E, F The level of miR-766-3p was examined using RT-qPCR in OA tissues (E) and IL-1β-treated CHON-001 cells (F). G The inhibitory effect of miR-766-3p inhibitor on miR-766-3p level was analyzed using RT-qPCR. H The expression of miR-766-3p was quantified using RT-qPCR after transfection of si-NC, si-circ-PDE1C, si-circ-PDE1C + inhibitor NC or si-circ-PDE1C + miR-766-3p inhibitor. ***P < 0.001

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Advances in Rheumatology

ISSN: 2523-3106