Fig. 2

IL-1β-induced cell apoptosis, inflammation and oxidative stress were alleviated with the knockdown of circ-PDE1C. A The circ-PDE1C expression was detected by RT-qPCR after CHON-001 cells were treated with IL-1β. B The circ-PDE1C and PDE1C mRNA levels were measured in si-NC or si-circ-PDE1C transfected CHON-001 cells. C Cell proliferation was assessed by CCK-8 assay in control, IL-1β, IL-1β + si-NC, and IL-1β + si-circ-PDE1C groups. D The apoptosis rate was determined by flow cytometry. E PCNA, Cleaved caspase-3 and Aggrecan protein levels were examined by western blotting. F TNF-α and IL-6 levels were assayed by ELISA. G–I Oxidative stress was evaluated by the detection of ROS (G), MDA (H) and SOD (I). **P < 0.01, ***P < 0.001