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Fig. 2 | Advances in Rheumatology

Fig. 2

From: Hck promotes IL-1β-induced extracellular matrix degradation, inflammation, and apoptosis in osteoarthritis via activation of the JAK-STAT3 signaling pathway

Fig. 2

Hck Knockdown Reduces ECM Degradation, Inflammation, and Apoptosis in Chondrocytes. A Hck level in chondrocytes treated with IL-1β, measured by RT-qPCR. B RT-qPCR confirmed the effectiveness of siRNA transfection in reducing Hck levels. C Cell viability was evaluated with CCK-8 test in IL-1β-treated chondrocytes following Hck knockdown. D-E ELISA measured IL-6 and TNF-α levels in IL-1β-treated chondrocytes following Hck knockdown. F Apoptosis in IL-1β-treated chondrocytes was assessed by flow cytometry analysis after Hck was silenced. G-H The levels of proteins related to apoptosis and ECM in IL-1β-treated chondrocytes following Hck knockdown was measured by western blot. P < 0.01 in contrast to the control group; ##P < 0.01 in contrast to the group receiving IL-1β

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Advances in Rheumatology

ISSN: 2523-3106