Fig. 2

CIA mice developed experimental arthritis and high disease severity. A Arthritis score and B Hind paw edema decrease over time in the tofacitinib-treated group compared to the vehicle group. C The mechanical threshold of nociception was decreased in both vehicle and tofacitinib groups compared to control mice. D Serum protein expression of IL-6 and E TNF-α from all experimental groups. F Histopathological score. Arrows indicate immune cell infiltration and bone/cartilage erosion. Representative data over time is related to established CIA at 50 days of disease. Data are presented as mean ± standard error of the mean (SEM). Statistical analysis between groups was performed using a two-way analysis of variance followed by Bonferroni’s test (Arthritis score, hind paw edema, nociception) and ANOVA one-way followed by Bonferroni’s test (Histopathological score, IL-.6, TNF-α). *p < 0.05; **p < 0.01; ***p < 0.0001. Control group n = 9; Vehicle group n = 10; Tofacitinib group n = 10