Fig. 3
From: The role of proteasome in muscle wasting of experimental arthritis
Proteasome-related gene expression levels were modulated by etanercept and methotrexate treatments. GA muscles of arthritic and healthy animals were used to extract mRNA and perform qRT-PCR for detection of A MuRF-1, B PSMβ5, C PSMβ8, D PSMβ6, E PSMβ9 subunits, and HPRT1 housekeeping gene. The mRNA expression is presented in fold change 2ΔCt versus values of CO group. CIA-Vehicle: CIA with PBS; CIA-ETN: CIA treated with etanercept; CIA-MTX: CIA treated with methotrexate. Data were analyzed by one-way ANOVA followed by Tukey’s test and are presented as mean ± SEM; n = 7 animals per group. *p < 0.05; **p < 0.01