Fig. 4
From: SIRT1 is transcriptionally repressed by YY1 and suppresses ferroptosis in rheumatoid arthritis
YY1 downregulated the expression of SIRT1 by inhibiting its transcription. Synoviocytes were transfected with pcDNA-3.1 or pcDNA-SIRT1. A JASPAR software predicted the potential binding site between YY1 and SIRT1. B ChIP detected the interaction between YY1 and SIRT1. C Dual-luciferase reporter assay detected the combination of YY1 and SIRT1. D The mRNA expression of SIRT1 was examined by qPCR. E The protein level of SIRT1 was examined by western blot. Experiments were carried out in triplicate, mean ± SD, **p < 0.01; ***p < 0.001